ABSTRACT
Two bacterial strains, i.e. Pseudomonas mendocina and Ochrobactnim sp. were isolated from bilge oil contaminated water of Mormugao harbour, Goa, India and grown in a culture medium with hexadecane as the sole carbon source. Pseudomonas mendocina was used in further studies as it was the dominant strain. This strain effectively degraded tetradecane, hexadecane and octadecane leaving a residual concentration of about 73%, 54% and 40% respectively in 120 h. Sequence analysis of the dominant bands from the denaturing gradient gel electrophoresis profiles revealed the differences between the genera of bilge oil contaminated sea water and its enrichment culture on hexadecane indicating a shift in community structure based on the type of substrate available. Pseudomonas mendocina amplified for the following catabolic genes namely C23O, nid and ndo. Based on the catabolic gene study the potential of the bacterial strain isolated, i.e. Pseudomonas mendocina seems to be interesting as it will be able to degrade polyaromatic hydrocarbons as well. Physicochemical properties of Pseudomonas mendocina indicates production of exopolysaccharides based on the value of its isoelectric point
Subject(s)
Pseudomonas mendocina , Ochrobactrum , Biodegradation, Environmental , Denaturing Gradient Gel Electrophoresis , Stearates , Cyclic AMP Receptor ProteinABSTRACT
The purpose of this work was to study the biodegradation of citronellol, citronellal and citronellyl acetate by a soil Pseudomonas mendocina strain (IBPse 105) isolated from a Cymbopogon windelandi field. This strain efficiently used citronellol, citronellal, citronellyl acetate and myrcene as sole source of carbon, but was not able to grow on other 15 monoterpenoids evaluated. Gas chromatography-mass spectrometry (GC-MS) analysis of metabolites accumulation during P. medocina IBPse 105 growth on citronellol showed that this strain uses the citronellol catabolic pathway described for other species of the genus. IBPse 105 degradation of citronellyl acetate initiates by its hydrolysis to citronellol. The mini-Tn5 insertion in mutant IBPse 105-303, impaired in citronellol degradation, but able to grow on citronellal, was located in a homologous of the P. aeruginosa atuB gene, that codifies citronellol deshydrogenase.
Subject(s)
Monoterpenes/metabolism , Pseudomonas mendocina/metabolism , Biotransformation , Gas Chromatography-Mass Spectrometry , Hydrolysis , Mutagenesis , Pseudomonas mendocina/genetics , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
In this study, we found that when Pseudomonas mendocina NK-01 accumulated intracellular carbon reserve, medium chain length poly (3-hydroxyalkanoates), it also synthesized extracellular saccharides, alginate oligosaccharides. The high carbon nitrogen ratio of culture medium facilitated alginate oligosaccharides production. We analyzed the structure of alginate oligosaccharide by Ultraviolet-Visible Spectrophotometry, Fourier Transform Infrared Spectroscopy, 1H and 13C of Nuclear Magnetic Resonance, and found that it was compounded in line from beta-D-mannuronic acids and alpha-L-gluronic acids via beta-(1-->4)/ alpha-(1-->4) bonds, which acetylated partly on the 2- and/or 3-hydroxy. In addition, we determined the weight-average molecular weight of alginate oligosaccharides by gel permeation chromatography to be 2054.
Subject(s)
Alginates , Chemistry , Glucuronic Acid , Chemistry , Genetics , Hexuronic Acids , Chemistry , Molecular Weight , Oligosaccharides , Chemistry , Genetics , Pseudomonas mendocina , MetabolismABSTRACT
Pseudomonas mendocina P2d grew in sodium benzoate at as high as 1% concentration and formed a quinonoid compound, identified as ortho-benzoquinone, that rendered the medium orange to wine-red in colour. The quinone was not metabilised further by the organism. Sodium benzoate was converted to catechol, which was a central metabolite forming ortho-benzoquinone and 2- hydroxymuconic semialdehyde (2-HMS) via. meta ring cleavage pathway.